P-109: A Histomorphometrical Study ofthe Protective Effect of Grape Seed Hydroalcholic Extract against Fluxetine Toxicosis in Testis Tissue of Male Mice

Background: Fluoxetine (FLX) belongs to the selective serotonin reuptake inhibitor group of antidepressants, which effectively alleviate symptoms of a wide spectrumof mood disorders. GSE contains mainly flavonoids, which involved in ameliorating the oxidative stress in vitro and in vivo. Thus this study was designed to investigate Protective effect of GSE hydroalcholic extract against fluoxetine-induced histomorphometrical damage on testicular. Materials and Methods: In present study, 24 mature male mice were used. The animals were divided into four groups as, control, FLX (20 mg/kg), FLX (20 mg/kg) + GSE (100 mg/kg) and GSE (100 mg/kg) orally for 42 days. after the last gavage, animals were sacrificed. The tissues were fixed in 10% formalin, processed and blocks were made in paraffin wax. 5-7 μm thick sections were cut and stained with haematoxylin and eosin. Diameter of the seminiferous tubules (DST), Seminiferous tubules epithelial height (GEH), Testicular capsule thickness (TC), interstitial testicular tissue thickness (ITT), were examined in the light microscope under high magnification (X 400) by Scaled lens. Results: Histomorphometrical results showed that the male mice exposed to fluxetine had significantly (p<0.05) reduced in (GEH) and significantly (p<0.05) increased in (ITT) in comparison with the control, GSE and FLX+ GSE groups. On the other hand (DST) and (TC) was not statically significant between all groups. Conclusion: Fluxetine can cause infertility in male mice, Grape seed extract combined with fluxetine can reduce the negative effects of fluxetine.